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Description

All mRNA levels were standardized to RPL32 with SYBR Green reagents (Thermo Fisher Scientific), and 384 well plates were loaded and sent to the University of Utah Genomics Core laboratories for qPCR

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The assay based on the reaction of GSH with 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) in the presence of glutathione reductase, was performed according to the protocol 56 and measured with a TECAN plate reader at 412 nm

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